Hotspot

psbE-petL

Genome · 78315-79480 · 1166 bp

Back to Diplopterygium

Pi 0.0111
MarkerSeek score 59.20
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
Genome
length_bp
1166
variable_sites
13
pi
0.0111
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 AATGTCAGCAAAGGGACGTT ACGTCATAGAAGCTGGATCT 45.0 45.0 58.0 55.8 1304-1310 bp 1.0 62.7
psbE-petL_p2 GGACGTTCTCCCGTATTTCC ACGTCATAGAAGCTGGATCT 55.0 45.0 58.1 55.8 1291-1297 bp 1.0 62.3
psbE-petL_p3 GCTATGGATGACCCGATGTC ACGTCATAGAAGCTGGATCT 55.0 45.0 57.9 55.8 1337-1343 bp 1.0 62.1
psbE-petL_p4 AATGTCAGCAAAGGGACGTT ACGTCATAGAAGCTGGATCTT 45.0 42.9 58.0 56.5 1304-1310 bp 1.0 61.0
psbE-petL_p5 CCAGCAATGAACAGAGAGGG ACGTCATAGAAGCTGGATCT 55.0 45.0 58.3 55.8 1366-1372 bp 1.0 60.8