Hotspot

accD-psaI

Genome · 71919-72507 · 589 bp

Back to Diplopterygium

Pi 0.0137

MarkerSeek score 63.50

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: Genome
length_bp: 589
variable_sites: 8
pi: 0.0137
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TCCACGTAATCTGATGAAAGGT	CCGAACCTACTAGGGGTACA	40.9	55.0	57.2	57.6	711-714 bp	1.0	55.1
accD-psaI_p2	TCCACGTAATCTGATGAAAGGT	ACCGAACCTACTAGGGGTAC	40.9	55.0	57.2	57.6	712-715 bp	1.0	55.1
accD-psaI_p3	CCACGTAATCTGATGAAAGGTG	CCGAACCTACTAGGGGTACA	45.5	55.0	57.1	57.6	710-713 bp	1.0	54.8
accD-psaI_p4	CCACGTAATCTGATGAAAGGTG	ACCGAACCTACTAGGGGTAC	45.5	55.0	57.1	57.6	711-714 bp	1.0	54.8
accD-psaI_p5	TTCCACGTAATCTGATGAAAGGT	CCGAACCTACTAGGGGTACA	39.1	55.0	57.8	57.6	712-715 bp	1.0	52.4