Hotspot

ndhC-trnV-UAC

Genome · 64710-65392 · 683 bp

Back to Diplopterygium

Pi 0.0117

MarkerSeek score 65.50

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: Genome
length_bp: 683
variable_sites: 8
pi: 0.0117
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	AGGCAAGGAATGAGGCTAGA	GCATGTTGGGTTCGTAAAGC	50.0	50.0	58.1	58.0	843 bp	1.0	79.7
ndhC-trnV-UAC_p2	AGGCAAGGAATGAGGCTAGA	GGTTCGAATCCGTATAGCCC	50.0	55.0	58.1	57.9	773 bp	1.0	79.3
ndhC-trnV-UAC_p3	AGGCAAGGAATGAGGCTAGA	ATATTCCGCTCTGCATGACC	50.0	50.0	58.1	57.8	805 bp	1.0	78.5
ndhC-trnV-UAC_p4	AGGCAAGGAATGAGGCTAGA	CCGAGAATGTCTACGGTTCG	50.0	55.0	58.1	58.2	787 bp	1.0	78.5
ndhC-trnV-UAC_p5	AGGCAAGGAATGAGGCTAGA	TCTGCATGACCGAGAATGTC	50.0	50.0	58.1	57.7	796 bp	1.0	77.5