Hotspot

rps12-trnV-GAC

Genome · 2741-6130 · 3390 bp

Back to Diplopterygium

Pi 0.00979
MarkerSeek score 53.50
Primer available yes
Label rps12-trnV-GAC

Feature Metadata

label_name
rps12-trnV-GAC
feature_type
igs
region
Genome
length_bp
3390
variable_sites
33
pi
0.00979
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
53.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps12-trnV-GAC_p1 CCATGGGTGACACAGATCAG TAGCCACAGGGTATACGCTT 55.0 50.0 58.0 58.2 205 bp 1.0 79.6
rps12-trnV-GAC_p2 CCATGGGTGACACAGATCAG GCCACAGGGTATACGCTTTT 55.0 50.0 58.0 58.3 203 bp 1.0 79.3
rps12-trnV-GAC_p3 GACACAGATCAGCCGAAGAG TAGCCACAGGGTATACGCTT 55.0 50.0 58.1 58.2 197 bp 1.0 79.3
rps12-trnV-GAC_p4 GACACAGATCAGCCGAAGAG GCCACAGGGTATACGCTTTT 55.0 50.0 58.1 58.3 195 bp 1.0 79.0
rps12-trnV-GAC_p5 ACAGATCAGCCGAAGAGAGT TAGCCACAGGGTATACGCTT 50.0 50.0 58.2 58.2 194 bp 1.0 78.7