Hotspot

psaC

SSC · 118631-118876 · 246 bp

Back to Diplolaena

Pi 0.00407

MarkerSeek score 45.20

Primer available yes

Label psaC

Feature Metadata

label_name: psaC
feature_type: gene
region: SSC
length_bp: 246
variable_sites: 1
pi: 0.00407
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC_p1	AAGTGGGTTTTTGGGAACGT	CGATGTTTCAAAATTGACTAGATCG	45.0	36.0	58.1	57.1	335 bp	1.0	40.2
psaC_p2	CAAGTGGGTTTTTGGGAACG	CGATGTTTCAAAATTGACTAGATCG	50.0	36.0	57.8	57.1	336 bp	1.0	40.2
psaC_p3	AAGTGGGTTTTTGGGAACGT	CGATGTTTCAAAATTGACTAGATCGA	45.0	34.6	58.1	58.3	335 bp	1.0	40.2
psaC_p4	CAAGTGGGTTTTTGGGAACG	CGATGTTTCAAAATTGACTAGATCGA	50.0	34.6	57.8	58.3	336 bp	1.0	40.2
psaC_p5	AAGTGGGTTTTTGGGAACGT	ACGATGTTTCAAAATTGACTAGA	45.0	30.4	58.1	54.6	336 bp	1.0	40.2