Hotspot

ndhD-psaC

SSC · 118436-118630 · 195 bp

Back to Diplolaena

Pi 0.103

MarkerSeek score 74.30

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 195
variable_sites: 20
pi: 0.103
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	TGTGGCTGAATAAAGGCGTT	CAAATTGCTTCCGCTCCAAG	45.0	50.0	58.1	58.0	797-798 bp	1.0	81.4
ndhD-psaC_p2	TTGTGGCTGAATAAAGGCGT	CAAATTGCTTCCGCTCCAAG	45.0	50.0	58.1	58.0	798-799 bp	1.0	81.4
ndhD-psaC_p3	TGTGGCTGAATAAAGGCGTT	GTGTTGGTTGTAAGCGATGC	45.0	50.0	58.1	58.0	766-767 bp	1.0	81.3
ndhD-psaC_p4	TTAAAGAACCCGCGGCTATC	CAAATTGCTTCCGCTCCAAG	50.0	50.0	58.1	58.0	364 bp	0.5	59.4
ndhD-psaC_p5	TTAAAGAACCCGCGGCTATC	GTGTTGGTTGTAAGCGATGC	50.0	50.0	58.1	58.0	333 bp	0.5	59.3