Hotspot

psbZ-trnG-GCC

LSC · 37600-37978 · 379 bp

Back to Diplolaena

Pi 0.0449
MarkerSeek score 68.30
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
379
variable_sites
17
pi
0.0449
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 AGTCTTTCTGGTGGGTATCCT TCTTCTCCTTGGCAAAGAGA 47.6 45.0 57.8 56.1 464 bp 1.0 57.9
psbZ-trnG-GCC_p2 TGGTACATCAGTGTGGATTGG TCTTCTCCTTGGCAAAGAGA 47.6 45.0 57.7 56.1 488 bp 1.0 56.6
psbZ-trnG-GCC_p3 AGTCTTTCTGGTGGGTATCCT TCTTCTCCTTGGCAAAGAGAA 47.6 42.9 57.8 56.9 464 bp 1.0 56.1
psbZ-trnG-GCC_p4 TGGTACATCAGTGTGGATTGG TCTTCTCCTTGGCAAAGAGAA 47.6 42.9 57.7 56.9 488 bp 1.0 54.8
psbZ-trnG-GCC_p5 GGTACATCAGTGTGGATTGGA TCTTCTCCTTGGCAAAGAGA 47.6 45.0 57.4 56.1 487 bp 1.0 54.4