Hotspot

trnS-UGA-psbZ

LSC · 37056-37410 · 355 bp

Back to Diplolaena

Pi 0.00845

MarkerSeek score 50.40

Primer available yes

Label trnS-UGA-psbZ

Feature Metadata

label_name: trnS-UGA-psbZ
feature_type: igs
region: LSC
length_bp: 355
variable_sites: 3
pi: 0.00845
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbZ_p1	CAAGACCGGAGCTATCAACC	CCGGAGAAGCAAATACAACG	55.0	50.0	58.1	57.2	481 bp	1.0	73.6
trnS-UGA-psbZ_p2	CAAGACCGGAGCTATCAACC	CGGAGAAGCAAATACAACGG	55.0	50.0	58.1	57.2	480 bp	1.0	73.6
trnS-UGA-psbZ_p3	TTCACCGAGCAAAAGGAGAG	CCGGAGAAGCAAATACAACG	50.0	50.0	57.8	57.2	551 bp	1.0	72.6
trnS-UGA-psbZ_p4	TTCACCGAGCAAAAGGAGAG	CGGAGAAGCAAATACAACGG	50.0	50.0	57.8	57.2	550 bp	1.0	72.6
trnS-UGA-psbZ_p5	CAAGACCGGAGCTATCAACC	TGCAAAAACAGCCAATTGGA	55.0	40.0	58.1	57.0	423 bp	1.0	72.1