Hotspot

trnY-GUA-trnE-UUC

LSC · 31971-32029 · 59 bp

Back to Diplolaena

Pi 0.0339

MarkerSeek score 63.40

Primer available yes

Label trnY-GUA-trnE-UUC

Feature Metadata

label_name: trnY-GUA-trnE-UUC
feature_type: igs
region: LSC
length_bp: 59
variable_sites: 2
pi: 0.0339
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY-GUA-trnE-UUC_p1	TGGATTTGAACCAGCGTAGG	AAGTATGCCCCCATCGTCTA	50.0	50.0	57.9	57.9	212 bp	1.0	79.1
trnY-GUA-trnE-UUC_p2	TGGATCGGCGAATTATTGGG	AAGTATGCCCCCATCGTCTA	50.0	50.0	58.1	57.9	238 bp	1.0	79.0
trnY-GUA-trnE-UUC_p3	ATTTGAACCAGCGTAGGCAT	AAGTATGCCCCCATCGTCTA	45.0	50.0	57.9	57.9	209 bp	1.0	79.0
trnY-GUA-trnE-UUC_p4	CATGGTGGATCGGCGAATTA	AAGTATGCCCCCATCGTCTA	50.0	50.0	58.1	57.9	243 bp	1.0	78.9
trnY-GUA-trnE-UUC_p5	TGGATTTGAACCAGCGTAGG	CTCTCTTTCAAGGAGGCAGC	50.0	55.0	57.9	58.3	178 bp	1.0	77.9