Hotspot

rpoB-trnC-GCA

LSC · 27352-28580 · 1229 bp

Back to Diplolaena

Pi 0.0149

MarkerSeek score 54.00

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1229
variable_sites: 18
pi: 0.0149
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 54.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	ATTCCCGCATTTCCATCTCC	GATCAAGCGACACCCAGATT	50.0	50.0	58.0	58.0	1308-1330 bp	1.0	82.1
rpoB-trnC-GCA_p2	ATTCCCGCATTTCCATCTCC	ACCAGCCAAGCAGAATAAGG	50.0	50.0	58.0	57.9	1351-1373 bp	1.0	81.2
rpoB-trnC-GCA_p3	ATTCCCGCATTTCCATCTCC	AAGCGACACCCAGATTTGAA	50.0	45.0	58.0	57.7	1304-1326 bp	1.0	80.2
rpoB-trnC-GCA_p4	ATTCCCGCATTTCCATCTCC	AAAAGGATTTGCAGTCCCCC	50.0	50.0	58.0	58.4	1277-1299 bp	1.0	79.6
rpoB-trnC-GCA_p5	GCATTTCCATCTCCGAGCAT	GATCAAGCGACACCCAGATT	50.0	50.0	58.4	58.0	1302-1324 bp	1.0	79.1