Hotspot

atpA-atpF

LSC · 11717-11777 · 61 bp

Back to Diplolaena

Pi 0.0492

MarkerSeek score 80.80

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 61
variable_sites: 3
pi: 0.0492
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	GTCGCCTACTTGAAGTACGG	ATTGGCATATTTGGGGCGAT	55.0	45.0	58.1	58.0	211-218 bp	1.0	80.3
atpA-atpF_p2	CCGTCGCCTACTTGAAGTAC	ATTGGCATATTTGGGGCGAT	55.0	45.0	58.1	58.0	213-220 bp	1.0	80.2
atpA-atpF_p3	ATTGCTCGATACGTTCACGG	ATTGGCATATTTGGGGCGAT	50.0	45.0	58.4	58.0	158-165 bp	1.0	77.8
atpA-atpF_p4	TCGCCTACTTGAAGTACGGT	ATTGGCATATTTGGGGCGAT	50.0	45.0	58.5	58.0	210-217 bp	1.0	77.3
atpA-atpF_p5	CGTCGCCTACTTGAAGTACG	ATTGGCATATTTGGGGCGAT	55.0	45.0	58.5	58.0	212-219 bp	1.0	77.3