Hotspot

trnG-UCC-trnR-UCU

LSC · 9730-9916 · 187 bp

Back to Diplolaena

Pi 0.0214
MarkerSeek score 64.80
Primer available yes
Label trnG-UCC-trnR-UCU

Feature Metadata

label_name
trnG-UCC-trnR-UCU
feature_type
igs
region
LSC
length_bp
187
variable_sites
4
pi
0.0214
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnR-UCU_p1 AGCCTTCCAAGCTAACGATG TGGAAATCAATTGCGTCCAA 50.0 40.0 58.0 56.2 314 bp 1.0 66.6
trnG-UCC-trnR-UCU_p2 CCTAGCCTTCCAAGCTAACG TGGAAATCAATTGCGTCCAA 55.0 40.0 58.1 56.2 317 bp 1.0 66.3
trnG-UCC-trnR-UCU_p3 AGCCTTCCAAGCTAACGATG ATGGAAATCAATTGCGTCCA 50.0 40.0 58.0 56.0 315 bp 1.0 64.7
trnG-UCC-trnR-UCU_p4 CCTAGCCTTCCAAGCTAACG ATGGAAATCAATTGCGTCCA 55.0 40.0 58.1 56.0 318 bp 1.0 64.5
trnG-UCC-trnR-UCU_p5 CCCTAGCCTTCCAAGCTAAC TGGAAATCAATTGCGTCCAA 55.0 40.0 57.7 56.2 318 bp 1.0 64.1