Hotspot

trnH-GUG-psbA

LSC · 100-480 · 381 bp

Back to Diplolaena

Pi 0.137

MarkerSeek score 64.60

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 381
variable_sites: 14
pi: 0.137
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.27
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	55.0	55.0	58.1	57.9	451 bp	0.5	58.4
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	465 bp	0.5	58.3
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	464 bp	0.5	58.3
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	466 bp	0.5	58.3
trnH-GUG-psbA_p5	CGCATGGTGGATTCACAATC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.5	57.9	480 bp	0.5	55.6