Hotspot

petB

Genome · 71090-72541 · 1452 bp

Back to Diplazium

Pi 0.0578

MarkerSeek score 80.50

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: Genome
length_bp: 1452
variable_sites: 145
pi: 0.0578
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	GGGGGTTCACGAATGGATAC	AAGGTCGGAAGAGAGACACA	55.0	50.0	58.0	58.0	1831-1837 bp	1.0	88.6
petB_p2	GGGGGTTCACGAATGGATAC	TCGGAAGAGAGACACACCTT	55.0	50.0	58.0	58.0	1827-1833 bp	1.0	88.6
petB_p3	GGATCTCTGAGCTCTTTGGC	AAGGTCGGAAGAGAGACACA	55.0	50.0	58.0	58.0	2294-2300 bp	1.0	88.4
petB_p4	CCTGATGGGACTTTCCATGG	TCGGAAGAGAGACACACCTT	55.0	50.0	57.9	58.0	1967-1973 bp	1.0	88.4
petB_p5	GGATCTCTGAGCTCTTTGGC	TCGGAAGAGAGACACACCTT	55.0	50.0	58.0	58.0	2290-2296 bp	1.0	88.3