Hotspot

ndhF

Genome · 106125-108356 · 2232 bp

Back to Diplazium

Pi 0.0721

MarkerSeek score 78.90

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: Genome
length_bp: 2232
variable_sites: 290
pi: 0.0721
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TCCCATTATAGGAGGAGGCG	CGGAGTTGTTTACCACCGAT	55.0	50.0	58.1	57.9	2913-2924 bp	1.0	87.1
ndhF_p2	AACTTCGCGTACACCCTAAC	CGGAGTTGTTTACCACCGAT	50.0	50.0	57.9	57.9	2933-2944 bp	1.0	87.1
ndhF_p3	AGGTAAACTTCGCGTACACC	CGGAGTTGTTTACCACCGAT	50.0	50.0	57.9	57.9	2938-2949 bp	1.0	87.1
ndhF_p4	GTAAACTTCGCGTACACCCT	CGGAGTTGTTTACCACCGAT	50.0	50.0	57.9	57.9	2936-2947 bp	1.0	87.1
ndhF_p5	CGTGAGGTAAACTTCGCGTA	CGGAGTTGTTTACCACCGAT	50.0	50.0	58.0	57.9	2942-2953 bp	0.75	76.2