Hotspot

ccsA-ndhD

Genome · 111780-112184 · 405 bp

Back to Diplazium

Pi 0.132

MarkerSeek score 77.70

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: Genome
length_bp: 405
variable_sites: 87
pi: 0.132
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TGCAGTCTCATTGGTCGATG	AGGAATGAGTGGTTTTGCGT	50.0	45.0	58.0	58.0	1299 bp	0.25	49.7
ccsA-ndhD_p2	TGCTTTGCAGTCTCATTGGT	AGGAATGAGTGGTTTTGCGT	45.0	45.0	58.0	58.0	1304 bp	0.25	49.7
ccsA-ndhD_p3	CTATGCTGACTCACGCCTTT	AGGAATGAGTGGTTTTGCGT	50.0	45.0	58.0	58.0	1374 bp	0.25	49.7
ccsA-ndhD_p4	TGCAGTCTCATTGGTCGATG	TCTCACGGCTTAATTGGAGC	50.0	50.0	58.0	58.0	1457 bp	0.25	49.7
ccsA-ndhD_p5	TGCTTTGCAGTCTCATTGGT	TCTCACGGCTTAATTGGAGC	45.0	50.0	58.0	58.0	1462 bp	0.25	49.7