Hotspot

trnP-GGG-trnL-UAG

Genome · 110177-110454 · 278 bp

Back to Diplazium

Pi 0.129

MarkerSeek score 81.30

Primer available yes

Label trnP-GGG-trnL-UAG

Feature Metadata

label_name: trnP-GGG-trnL-UAG
feature_type: igs
region: Genome
length_bp: 278
variable_sites: 60
pi: 0.129
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-GGG-trnL-UAG_p1	GCTACCAAACTGCGCCATAT	CTCTGGCACTGCTTCCTAAG	50.0	55.0	58.7	58.0	347-357 bp	1.0	85.9
trnP-GGG-trnL-UAG_p2	GCTACCAAACTGCGCCATAT	CTGGCACTGCTTCCTAAGAG	50.0	55.0	58.7	58.0	345-355 bp	1.0	85.9
trnP-GGG-trnL-UAG_p3	GCTACCAAACTGCGCCATAT	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.7	57.9	334-344 bp	1.0	85.2
trnP-GGG-trnL-UAG_p4	GACCTCCATCACCCCAAGAT	CTGGCACTGCTTCCTAAGAG	55.0	55.0	58.8	58.0	370-380 bp	1.0	85.0
trnP-GGG-trnL-UAG_p5	GACCTCCATCACCCCAAGAT	CTCTGGCACTGCTTCCTAAG	55.0	55.0	58.8	58.0	372-382 bp	1.0	84.9