Hotspot

trnT-UGU-trnR-ACG

Genome · 84442-85215 · 774 bp

Back to Diplazium

Pi 0.048

MarkerSeek score 83.00

Primer available yes

Label trnT-UGU-trnR-ACG

Feature Metadata

label_name: trnT-UGU-trnR-ACG
feature_type: igs
region: Genome
length_bp: 774
variable_sites: 64
pi: 0.048
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 83.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnR-ACG_p1	AGTACGGAGGTTCGAATCCT	CTCAGGGGATTAGAGCACGT	50.0	55.0	57.9	58.9	826-869 bp	1.0	81.8
trnT-UGU-trnR-ACG_p2	AGTACGGAGGTTCGAATCCT	TCAGGGGATTAGAGCACGTG	50.0	55.0	57.9	59.2	825-868 bp	1.0	79.5
trnT-UGU-trnR-ACG_p3	AGTACGGAGGTTCGAATCCT	TCAGGGGATTAGAGCACGT	50.0	52.6	57.9	57.7	825-868 bp	1.0	78.5
trnT-UGU-trnR-ACG_p4	AGTACGGAGGTTCGAATCCT	CAGGGGATTAGAGCACGTG	50.0	57.9	57.9	57.6	824-867 bp	1.0	78.0
trnT-UGU-trnR-ACG_p5	CGTAGTACGGAGGTTCGAATC	CTCAGGGGATTAGAGCACGT	52.4	55.0	58.0	58.9	829-872 bp	1.0	74.8