Hotspot

petA-psbJ

Genome · 59617-60511 · 895 bp

Back to Diplazium

Pi 0.103

MarkerSeek score 83.10

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: Genome
length_bp: 895
variable_sites: 161
pi: 0.103
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 83.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGTTGGTGGATTTGGTCAGG	TTCCCTCGTGGTTGATAGGT	50.0	50.0	58.0	58.1	1162 bp	0.25	49.5
petA-psbJ_p2	TAATCGCATTCCAGCCGAAA	TTCCCTCGTGGTTGATAGGT	45.0	50.0	58.0	58.1	1603 bp	0.25	49.3
petA-psbJ_p3	GAAGTTGGGGAAGTTGTCGT	TTCCCTCGTGGTTGATAGGT	50.0	50.0	58.0	58.1	1576 bp	0.25	49.3
petA-psbJ_p4	TGGGGAAGTTGTCGTTTCAG	TTCCCTCGTGGTTGATAGGT	50.0	50.0	58.0	58.1	1571 bp	0.25	49.3
petA-psbJ_p5	GCCGATCAGCCATTGACTAA	TTCCCTCGTGGTTGATAGGT	50.0	50.0	58.0	58.1	1191 bp	0.25	49.2