Hotspot

rpoB-trnD-GUC

Genome · 26507-27274 · 768 bp

Back to Diplazium

Pi 0.0948

MarkerSeek score 80.60

Primer available yes

Label rpoB-trnD-GUC

Feature Metadata

label_name: rpoB-trnD-GUC
feature_type: igs
region: Genome
length_bp: 768
variable_sites: 122
pi: 0.0948
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnD-GUC_p1	CAACCCCCTCTTGCTTCTTT	CGGTAACTCGATGAGGCAAA	50.0	50.0	58.0	58.0	1643 bp	0.25	50.0
rpoB-trnD-GUC_p2	CAAGTTCTGGTAGTACGGGC	CGGTAACTCGATGAGGCAAA	55.0	50.0	58.0	58.0	1046 bp	0.25	49.9
rpoB-trnD-GUC_p3	GCAAGTTCTGGTAGTACGGG	CGGTAACTCGATGAGGCAAA	55.0	50.0	58.0	58.0	1047 bp	0.25	49.9
rpoB-trnD-GUC_p4	CAACCCCCTCTTGCTTCTTT	AGCGCATCTCATTCGAGTTT	50.0	45.0	58.0	58.0	1483 bp	0.25	49.9
rpoB-trnD-GUC_p5	CAACCCCCTCTTGCTTCTTT	ATGAGGCAAAACCGAAGTGT	50.0	45.0	58.0	58.0	1633 bp	0.25	49.8