Hotspot

ndhF

Genome · 106573-108792 · 2220 bp

Back to Diplaziopsis

Pi 0.0423

MarkerSeek score 69.30

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: Genome
length_bp: 2220
variable_sites: 94
pi: 0.0423
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TCCCATTATAGGAGGAGGCG	TCGGGCGTCTCTAAATGTTG	55.0	50.0	58.1	58.0	2755-2778 bp	1.0	82.7
ndhF_p2	GTCGGGAGAAATGAACACGA	TCGGGCGTCTCTAAATGTTG	50.0	50.0	57.9	58.0	2470-2494 bp	1.0	82.7
ndhF_p3	AGTCGGGAGAAATGAACACG	TCGGGCGTCTCTAAATGTTG	50.0	50.0	57.9	58.0	2471-2495 bp	1.0	82.6
ndhF_p4	TCCCATTATAGGAGGAGGCG	TGAGTAAGTATCTCGGGCGT	55.0	50.0	58.1	58.0	2767-2790 bp	1.0	82.3
ndhF_p5	GTCGGGAGAAATGAACACGA	TGAGTAAGTATCTCGGGCGT	50.0	50.0	57.9	58.0	2482-2506 bp	1.0	82.3