Hotspot

rrn5-trnR-ACG

Genome · 148872-149144 · 273 bp

Back to Diplaziopsis

Pi 0.0476

MarkerSeek score 73.90

Primer available yes

Label rrn5-trnR-ACG

Feature Metadata

label_name: rrn5-trnR-ACG
feature_type: igs
region: Genome
length_bp: 273
variable_sites: 13
pi: 0.0476
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn5-trnR-ACG_p1	GGTAACAATACTGCAGGGGG	TAGGGATGATTGATTGCGGG	55.0	50.0	57.9	57.4	415-420 bp	1.0	77.5
rrn5-trnR-ACG_p2	GAACCACACCGATCCATCTC	TAGGGATGATTGATTGCGGG	55.0	50.0	58.1	57.4	460-465 bp	1.0	77.2
rrn5-trnR-ACG_p3	CCGATCCATCTCGAACTTGG	TAGGGATGATTGATTGCGGG	55.0	50.0	58.1	57.4	452-457 bp	1.0	76.6
rrn5-trnR-ACG_p4	CGGTAACAATACTGCAGGGG	TAGGGATGATTGATTGCGGG	55.0	50.0	58.3	57.4	416-421 bp	1.0	75.3
rrn5-trnR-ACG_p5	CCTGCGGAAAAATAGCTCGA	TAGGGATGATTGATTGCGGG	50.0	50.0	58.3	57.4	392-397 bp	1.0	75.3