Hotspot

trnP-GGG-trnL-UAG

Genome · 110539-110799 · 261 bp

Back to Diplaziopsis

Pi 0.101

MarkerSeek score 78.20

Primer available yes

Label trnP-GGG-trnL-UAG

Feature Metadata

label_name: trnP-GGG-trnL-UAG
feature_type: igs
region: Genome
length_bp: 261
variable_sites: 26
pi: 0.101
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-GGG-trnL-UAG_p1	GCTACCAAACTGCGCCATAT	CTCTGGCACTGCTTCCTAAG	50.0	55.0	58.7	58.0	338-347 bp	1.0	81.3
trnP-GGG-trnL-UAG_p2	GCTACCAAACTGCGCCATAT	CTGGCACTGCTTCCTAAGAG	50.0	55.0	58.7	58.0	336-345 bp	1.0	81.3
trnP-GGG-trnL-UAG_p3	GCTACCAAACTGCGCCATAT	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.7	57.9	325-334 bp	1.0	80.8
trnP-GGG-trnL-UAG_p4	GACCTCCATCACCCCAAGAT	CTCTGGCACTGCTTCCTAAG	55.0	55.0	58.8	58.0	363-372 bp	1.0	80.1
trnP-GGG-trnL-UAG_p5	GACCTCCATCACCCCAAGAT	CTGGCACTGCTTCCTAAGAG	55.0	55.0	58.8	58.0	361-370 bp	1.0	80.1