Hotspot

rpl23-trnI-CAU

Genome · 82645-82806 · 162 bp

Back to Diplaziopsis

Pi 0.0864
MarkerSeek score 76.40
Primer available yes
Label rpl23-trnI-CAU

Feature Metadata

label_name
rpl23-trnI-CAU
feature_type
igs
region
Genome
length_bp
162
variable_sites
14
pi
0.0864
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
76.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl23-trnI-CAU_p1 GGTTTTGCTGCAACAAACGA TGGCGAATTCACAGGTTCAA 45.0 45.0 58.4 58.0 258 bp 1.0 81.2
rpl23-trnI-CAU_p2 GGTTTTGCTGCAACAAACGA TTGGCGAATTCACAGGTTCA 45.0 45.0 58.4 58.0 259 bp 1.0 81.2
rpl23-trnI-CAU_p3 TGGTTTTGCTGCAACAAACG TGGCGAATTCACAGGTTCAA 45.0 45.0 58.6 58.0 259 bp 1.0 79.0
rpl23-trnI-CAU_p4 TGGTTTTGCTGCAACAAACG TTGGCGAATTCACAGGTTCA 45.0 45.0 58.6 58.0 260 bp 1.0 78.9
rpl23-trnI-CAU_p5 GGTTTTGCTGCAACAAACGA GGCGAATTCACAGGTTCAAC 45.0 50.0 58.4 57.7 257 bp 1.0 78.8