Hotspot

psbE-petL

Genome · 61734-62493 · 760 bp

Back to Diplaziopsis

Pi 0.049

MarkerSeek score 77.60

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: Genome
length_bp: 760
variable_sites: 37
pi: 0.049
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	GCGTTCTCCTGTATTCCCAG	GTCAAAAAGGCGAAGTGACT	55.0	45.0	58.1	56.6	816-819 bp	1.0	72.3
psbE-petL_p2	GCGTTCTCCTGTATTCCCAG	GTCAAAAAGGCGAAGTGACTT	55.0	42.9	58.1	57.3	816-819 bp	1.0	70.1
psbE-petL_p3	AAAGGGGCGTTCTCCTGTAT	GTCAAAAAGGCGAAGTGACT	50.0	45.0	58.7	56.6	822-825 bp	1.0	67.0
psbE-petL_p4	AAGGGGCGTTCTCCTGTATT	GTCAAAAAGGCGAAGTGACT	50.0	45.0	58.7	56.6	821-824 bp	1.0	67.0
psbE-petL_p5	AATGATGTCAGCAAAGGGGC	GTCAAAAAGGCGAAGTGACT	50.0	45.0	58.8	56.6	834-837 bp	1.0	66.2