Hotspot

petA-psbJ

Genome · 60120-60972 · 853 bp

Back to Diplaziopsis

Pi 0.061

MarkerSeek score 77.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: Genome
length_bp: 853
variable_sites: 52
pi: 0.061
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GTTGCGTATCCAAGGTCTCA	GGGGTCGTCTCCTCGATAA	50.0	57.9	57.6	57.9	987-989 bp	1.0	72.4
petA-psbJ_p2	TTGCATCGGTTGTATTGGCA	GGGGTCGTCTCCTCGATAA	45.0	57.9	58.5	57.9	958-960 bp	1.0	71.9
petA-psbJ_p3	TTTGCATCGGTTGTATTGGC	GGGGTCGTCTCCTCGATAA	45.0	57.9	57.4	57.9	959-961 bp	1.0	70.4
petA-psbJ_p4	CGTTGCGTATCCAAGGTCTC	GGGGTCGTCTCCTCGATAA	55.0	57.9	58.7	57.9	988-990 bp	1.0	69.7
petA-psbJ_p5	GTTGCGTATCCAAGGTCTCA	TCTTACGGGGCTTACTCGG	50.0	57.9	57.6	58.5	1010-1012 bp	1.0	69.1