Hotspot

trnF-GAA-ndhJ

Genome · 46994-47246 · 253 bp

Back to Diplaziopsis

Pi 0.0576

MarkerSeek score 76.80

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: Genome
length_bp: 253
variable_sites: 14
pi: 0.0576
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TTGGATAGGGTGGCCTCTAC	50.0	55.0	58.1	58.2	347-357 bp	1.0	83.5
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	GTTGGATAGGGTGGCCTCTA	50.0	55.0	58.1	58.2	348-358 bp	1.0	83.4
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	GAAAGTTGGATAGGGTGGCC	50.0	55.0	58.1	58.2	352-362 bp	1.0	83.1
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	GATAGGGTGGCCTCTACGTA	50.0	55.0	58.1	57.7	344-354 bp	1.0	82.8
trnF-GAA-ndhJ_p5	AATGAGTTGGCCGAGATAGC	TTGGATAGGGTGGCCTCTAC	50.0	55.0	57.8	58.2	395-405 bp	1.0	81.2