Hotspot

trnS-UGA-psbC

Genome · 31738-32024 · 287 bp

Back to Diplaziopsis

Pi 0.0523

MarkerSeek score 68.30

Primer available yes

Label trnS-UGA-psbC

Feature Metadata

label_name: trnS-UGA-psbC
feature_type: igs
region: Genome
length_bp: 287
variable_sites: 15
pi: 0.0523
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbC_p1	GGGTTCGAATCCCTCTCTCT	TGTCTATGACACCCCTTAATTGA	55.0	39.1	58.2	57.0	333-354 bp	1.0	49.5
trnS-UGA-psbC_p2	GGGTTCGAATCCCTCTCTCT	TGTCTATGACACCCCTTAATTG	55.0	40.9	58.2	55.5	333-354 bp	1.0	45.9
trnS-UGA-psbC_p3	GGGTTCGAATCCCTCTCTCT	GTCTATGACACCCCTTAATTGA	55.0	40.9	58.2	55.2	332-353 bp	1.0	43.8
trnS-UGA-psbC_p4	TTCGAATCCCTCTCTCTCCT	TGTCTATGACACCCCTTAATTGA	50.0	39.1	56.9	57.0	330-351 bp	1.0	43.5
trnS-UGA-psbC_p5	GTTCGAATCCCTCTCTCTCC	TGTCTATGACACCCCTTAATTGA	55.0	39.1	56.8	57.0	331-352 bp	1.0	43.5