Hotspot

trnC-GCA-trnG-GCC

Genome · 30302-30731 · 430 bp

Back to Diplaziopsis

Pi 0.0896

MarkerSeek score 78.20

Primer available yes

Label trnC-GCA-trnG-GCC

Feature Metadata

label_name: trnC-GCA-trnG-GCC
feature_type: igs
region: Genome
length_bp: 430
variable_sites: 38
pi: 0.0896
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-trnG-GCC_p1	GATTTGCAGTCCTCCGTCTT	GTTCGATTCCCGCTATCCG	50.0	57.9	57.9	58.2	490-497 bp	1.0	76.8
trnC-GCA-trnG-GCC_p2	GATTTGCAGTCCTCCGTCTT	GGGTTCGATTCCCGCTATC	50.0	57.9	57.9	57.8	492-499 bp	1.0	76.6
trnC-GCA-trnG-GCC_p3	GATTTGCAGTCCTCCGTCTT	GGTTCGATTCCCGCTATCC	50.0	57.9	57.9	57.8	491-498 bp	1.0	76.6
trnC-GCA-trnG-GCC_p4	TCCGTCTTACCGCTTGACTA	GTTCGATTCCCGCTATCCG	50.0	57.9	58.2	58.2	478-485 bp	1.0	76.4
trnC-GCA-trnG-GCC_p5	TCCGTCTTACCGCTTGACTA	GGTTCGATTCCCGCTATCC	50.0	57.9	58.2	57.8	479-486 bp	1.0	76.2