Hotspot

trnY-GUA-trnE-UUC

Genome · 27637-27776 · 140 bp

Back to Diplaziopsis

Pi 0.0571

MarkerSeek score 75.00

Primer available yes

Label trnY-GUA-trnE-UUC

Feature Metadata

label_name: trnY-GUA-trnE-UUC
feature_type: igs
region: Genome
length_bp: 140
variable_sites: 8
pi: 0.0571
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY-GUA-trnE-UUC_p1	GTCCCCATTAACCACTCGAG	ATAAAAGGTAATCGGGCCCC	55.0	50.0	57.7	57.3	257 bp	1.0	74.6
trnY-GUA-trnE-UUC_p2	CCCATTAACCACTCGAGCAT	ATAAAAGGTAATCGGGCCCC	50.0	50.0	57.7	57.3	254 bp	1.0	74.5
trnY-GUA-trnE-UUC_p3	CTGGATTCGAACCAGCGTAG	ATAAAAGGTAATCGGGCCCC	55.0	50.0	58.4	57.3	301 bp	1.0	73.3
trnY-GUA-trnE-UUC_p4	TTGCCAGCGGATTTACAGTC	ATAAAAGGTAATCGGGCCCC	50.0	50.0	58.6	57.3	278 bp	1.0	72.5
trnY-GUA-trnE-UUC_p5	TATCAACCTACAGCCTCGGG	ATAAAAGGTAATCGGGCCCC	55.0	50.0	58.6	57.3	332 bp	1.0	71.8