Hotspot

psbK-psbI

Genome · 7239-7783 · 545 bp

Back to Diplaziopsis

Pi 0.0516

MarkerSeek score 75.60

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: Genome
length_bp: 545
variable_sites: 28
pi: 0.0516
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CAAGCCGCAGTTAGTTTTCG	ACGAAGAATATCACTACTGCGT	50.0	40.9	58.0	57.4	616-637 bp	1.0	62.4
psbK-psbI_p2	CAAGCCGCAGTTAGTTTTCG	GCGTAGACAAATAGTTTGAGGG	50.0	45.5	58.0	57.1	598-619 bp	1.0	60.7
psbK-psbI_p3	GCAAGCCGCAGTTAGTTTTC	ACGAAGAATATCACTACTGCGT	50.0	40.9	58.3	57.4	617-638 bp	1.0	60.2
psbK-psbI_p4	TTCTTCTGGCCTTCGTTTGG	ACGAAGAATATCACTACTGCGT	50.0	40.9	58.4	57.4	636-657 bp	1.0	59.5
psbK-psbI_p5	TGCCTGTAATACCGGTGTTC	ACGAAGAATATCACTACTGCGT	50.0	40.9	57.6	57.4	660-681 bp	1.0	59.4