Hotspot

ycf1

SSC · 123341-128902 · 5562 bp

Back to Diplacus

Pi 0.00378

MarkerSeek score 45.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5562
variable_sites: 65
pi: 0.00378
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCGATATGGGACGTTTTAGCA	AGTCGATATTGAAACTTGGATGA	42.9	34.8	57.2	55.8	197 bp	1.0	42.0
ycf1_p2	TCCAAATTCGATATGGGACGT	AGTCGATATTGAAACTTGGATGA	42.9	34.8	57.2	55.8	204 bp	1.0	42.0
ycf1_p3	TCGATATGGGACGTTTTAGCA	GTCGATATTGAAACTTGGATGATGA	42.9	36.0	57.2	57.5	196 bp	1.0	42.0
ycf1_p4	TCGATATGGGACGTTTTAGCA	AGTCGATATTGAAACTTGGATGATG	42.9	36.0	57.2	57.5	197 bp	1.0	42.0
ycf1_p5	TCCAAATTCGATATGGGACGT	GTCGATATTGAAACTTGGATGATGA	42.9	36.0	57.2	57.5	203 bp	1.0	42.0