Hotspot

ndhF

SSC · 109920-112172 · 2253 bp

Back to Diplacus

Pi 0.00398

MarkerSeek score 43.10

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2253
variable_sites: 27
pi: 0.00398
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AGCCCCCTTTTTGAAGAGTC	CGGTGAATCGGAAACAATTA	50.0	40.0	57.7	54.0	2341-2353 bp	1.0	47.2
ndhF_p2	GCCCCCTTTTTGAAGAGTCT	CGGTGAATCGGAAACAATTA	50.0	40.0	57.7	54.0	2340-2352 bp	1.0	47.2
ndhF_p3	AGCCCCCTTTTTGAAGAGTC	CGGTGAATCGGAAACAATTAA	50.0	38.1	57.7	54.9	2341-2353 bp	1.0	46.0
ndhF_p4	GCCCCCTTTTTGAAGAGTCT	CGGTGAATCGGAAACAATTAA	50.0	38.1	57.7	54.9	2340-2352 bp	1.0	46.0
ndhF_p5	AAGCCCCCTTTTTGAAGAGT	CGGTGAATCGGAAACAATTA	45.0	40.0	57.2	54.0	2342-2354 bp	1.0	43.4