Hotspot

accD

LSC · 57601-59118 · 1518 bp

Back to Diplacus

Pi 0.00261

MarkerSeek score 51.30

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1518
variable_sites: 10
pi: 0.00261
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	AGTGAAGTCTTACCCCTGGA	GGTCACCAAAGAAAGCTCCA	50.0	50.0	57.3	58.0	1721 bp	1.0	74.6
accD_p2	ACCCCTGGATTTCCACAAAA	GGTCACCAAAGAAAGCTCCA	45.0	50.0	57.2	58.0	1710 bp	1.0	74.0
accD_p3	AGTGAAGTCTTACCCCTGGA	AGGTCACCAAAGAAAGCTCC	50.0	50.0	57.3	57.7	1722 bp	1.0	72.5
accD_p4	GTCTTACCCCTGGATTTCCAC	GGTCACCAAAGAAAGCTCCA	52.4	50.0	58.0	58.0	1715 bp	1.0	72.3
accD_p5	ACCCCTGGATTTCCACAAAA	AGGTCACCAAAGAAAGCTCC	45.0	50.0	57.2	57.7	1711 bp	1.0	72.0