Hotspot

psaC-ndhE

SSC · 116901-117163 · 263 bp

Back to Diplacus

Pi 0.00541

MarkerSeek score 49.00

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 263
variable_sites: 4
pi: 0.00541
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 49.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	TACATCCGTCCCAGGGTATC	CTATTGGGCCGGCTATTGTT	55.0	50.0	58.0	58.0	437-449 bp	1.0	82.7
psaC-ndhE_p2	AATACATCGGTGGGACATGC	CTATTGGGCCGGCTATTGTT	50.0	50.0	58.0	58.0	411-423 bp	1.0	82.7
psaC-ndhE_p3	TCCGTCCCAGGGTATCATTT	CTATTGGGCCGGCTATTGTT	50.0	50.0	58.1	58.0	433-445 bp	1.0	81.9
psaC-ndhE_p4	ATCCGTCCCAGGGTATCATT	CTATTGGGCCGGCTATTGTT	50.0	50.0	57.9	58.0	434-446 bp	1.0	81.8
psaC-ndhE_p5	TTTACATCCGTCCCAGGGTA	CTATTGGGCCGGCTATTGTT	50.0	50.0	57.7	58.0	439-451 bp	1.0	80.7