Hotspot

psbB-psbT

LSC · 74106-74296 · 191 bp

Back to Diplacus

Pi 0.00262

MarkerSeek score 49.30

Primer available yes

Label psbB-psbT

Feature Metadata

label_name: psbB-psbT
feature_type: igs
region: LSC
length_bp: 191
variable_sites: 2
pi: 0.00262
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbT_p1	GGCATTCCAAAAACTTGGGG	TGGAACTTTAGGTGGTTCTCG	50.0	47.6	57.8	57.6	320-335 bp	1.0	70.3
psbB-psbT_p2	GGGCATTCCAAAAACTTGGG	TGGAACTTTAGGTGGTTCTCG	50.0	47.6	57.8	57.6	321-336 bp	1.0	70.3
psbB-psbT_p3	GGGGCATTCCAAAAACTTGG	TGGAACTTTAGGTGGTTCTCG	50.0	47.6	57.8	57.6	322-337 bp	1.0	70.2
psbB-psbT_p4	CGCTCAAGTAGAGTTTGGGG	TGGAACTTTAGGTGGTTCTCG	55.0	47.6	58.3	57.6	338-353 bp	1.0	69.4
psbB-psbT_p5	ACGCTCAAGTAGAGTTTGGG	TGGAACTTTAGGTGGTTCTCG	50.0	47.6	57.5	57.6	339-354 bp	1.0	67.9