Hotspot

ndhC-trnV-UAC

LSC · 50830-51751 · 922 bp

Back to Diplacus

Pi 0.00395
MarkerSeek score 44.40
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
922
variable_sites
12
pi
0.00395
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
44.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCATATTCGTAAAGCAAAAACA AGAAGGTCTACGGTTCGAGT 27.3 50.0 52.7 57.8 969-997 bp 1.0 41.7
ndhC-trnV-UAC_p2 TCATATTCGTAAAGCAAAAACA GAAGGTCTACGGTTCGAGTC 27.3 55.0 52.7 57.5 968-996 bp 1.0 41.7
ndhC-trnV-UAC_p3 ATCATATTCGTAAAGCAAAAACA AGAAGGTCTACGGTTCGAGT 26.1 50.0 53.3 57.8 970-998 bp 1.0 41.7
ndhC-trnV-UAC_p4 TCATATTCGTAAAGCAAAAACAT AGAAGGTCTACGGTTCGAGT 26.1 50.0 53.3 57.8 969-997 bp 1.0 41.7
ndhC-trnV-UAC_p5 TCATATTCGTAAAGCAAAAACA GTTCGAGTCCGTATAGCCCT 27.3 55.0 52.7 58.7 957-985 bp 1.0 41.7