Hotspot

psaA-ycf3

LSC · 42266-43021 · 756 bp

Back to Diplacus

Pi 0.0043

MarkerSeek score 54.20

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 756
variable_sites: 9
pi: 0.0043
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 54.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TGAGAAATGACCGGGTTTGG	TTGAAGATCACGAGGCGTTT	50.0	45.0	58.1	57.8	876-887 bp	1.0	79.8
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	TTGAAGATCACGAGGCGTTT	50.0	45.0	57.9	57.8	857-868 bp	1.0	79.7
psaA-ycf3_p3	TGAGAAATGACCGGGTTTGG	TTGGTTGAAGATCACGAGGC	50.0	50.0	58.1	58.2	880-891 bp	1.0	79.6
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	TTGGTTGAAGATCACGAGGC	50.0	50.0	57.9	58.2	861-872 bp	1.0	79.5
psaA-ycf3_p5	TGAGAAATGACCGGGTTTGG	AGCCGCTGAGTATTGGAAAC	50.0	50.0	58.1	58.3	943-954 bp	1.0	78.9