Hotspot

rps2-rpoC2

LSC · 16975-17192 · 218 bp

Back to Diplacus

Pi 0.00754

MarkerSeek score 51.40

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 218
variable_sites: 5
pi: 0.00754
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	CCCAAAATGAACTCCTGCCT	TTTGAGGGGGAAATGAGAGA	50.0	45.0	58.1	55.7	397-399 bp	1.0	62.6
rps2-rpoC2_p2	CCCAAAATGAACTCCTGCCT	TTGAGGGGGAAATGAGAGAT	50.0	45.0	58.1	55.5	396-398 bp	1.0	60.7
rps2-rpoC2_p3	CCTCCAGCATCTCTTCCAAG	TTTGAGGGGGAAATGAGAGA	55.0	45.0	57.7	55.7	380-382 bp	1.0	60.6
rps2-rpoC2_p4	CCCAAAATGAACTCCTGCCT	TTTGAGGGGGAAATGAGAGAT	50.0	42.9	58.1	56.3	397-399 bp	1.0	59.3
rps2-rpoC2_p5	CCCAAAATGAACTCCTGCCT	ATTTGAGGGGGAAATGAGAGA	50.0	42.9	58.1	56.3	398-400 bp	1.0	59.2