Hotspot

atpH-atpI

LSC · 14307-15300 · 994 bp

Back to Diplacus

Pi 0.00471

MarkerSeek score 51.50

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 994
variable_sites: 13
pi: 0.00471
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 51.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AACGGAAGCAGCAGAAATCA	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.1	57.9	1078-1079 bp	1.0	79.6
atpH-atpI_p2	AACGGAAGCAGCAGAAATCA	TCCTGTCATGTTCCTCGGAT	45.0	50.0	58.1	58.1	1126-1127 bp	1.0	79.5
atpH-atpI_p3	AGCCAATCCAGCAGCAATAA	TTTTGCAACTTTAGCTGCGG	45.0	45.0	57.9	57.9	1096-1097 bp	1.0	79.2
atpH-atpI_p4	AGCCAATCCAGCAGCAATAA	TCCTGTCATGTTCCTCGGAT	45.0	50.0	57.9	58.1	1144-1145 bp	1.0	79.1
atpH-atpI_p5	GCCAATCCAGCAGCAATAAC	TTTTGCAACTTTAGCTGCGG	50.0	45.0	57.8	57.9	1095-1096 bp	1.0	78.7