Hotspot

ndhD-psaC

SSC · 119760-120252 · 493 bp

Back to Diphylleia

Pi 0.0189

MarkerSeek score 57.60

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 493
variable_sites: 14
pi: 0.0189
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	TTTGACCGCTGTACATTGCT	TATGGCATGAGACAACTCGC	45.0	50.0	58.1	58.1	559-571 bp	1.0	81.1
ndhD-psaC_p2	ACCGCTGTACATTGCTAACA	TATGGCATGAGACAACTCGC	45.0	50.0	57.5	58.1	555-567 bp	1.0	78.2
ndhD-psaC_p3	TTTGACCGCTGTACATTGCT	ATGAGACAACTCGCAGCATG	45.0	50.0	58.1	58.6	553-565 bp	1.0	76.5
ndhD-psaC_p4	TTTGACCGCTGTACATTGCT	CATGAGACAACTCGCAGCAT	45.0	50.0	58.1	58.6	554-566 bp	1.0	76.5
ndhD-psaC_p5	TGACCGCTGTACATTGCTAA	TATGGCATGAGACAACTCGC	45.0	50.0	57.2	58.1	557-569 bp	1.0	75.9