Hotspot

rpl32-trnL-UAG

SSC · 116403-117241 · 839 bp

Back to Diphylleia

Pi 0.0169

MarkerSeek score 62.70

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 839
variable_sites: 21
pi: 0.0169
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GTGGCAGTAAAAGCGCTTTC	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.3	58.0	946-958 bp	1.0	80.3
rpl32-trnL-UAG_p2	GTGGCAGTAAAAGCGCTTTC	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.3	58.0	947-959 bp	1.0	80.3
rpl32-trnL-UAG_p3	TGGCAGTAAAAGCGCTTTCT	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.4	58.0	945-957 bp	1.0	79.6
rpl32-trnL-UAG_p4	TGGCAGTAAAAGCGCTTTCT	CAGCGTGTCTACCGATTTCA	45.0	50.0	58.4	58.0	946-958 bp	1.0	79.6
rpl32-trnL-UAG_p5	GTGGCAGTAAAAGCGCTTTC	GTTCTTTTCGAAGATGCCGC	50.0	50.0	58.3	58.1	1011-1023 bp	1.0	79.2