Hotspot

trnL-UAA-trnF-GAA

LSC · 49979-50365 · 387 bp

Back to Diphylleia

Pi 0.0121

MarkerSeek score 61.90

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 387
variable_sites: 7
pi: 0.0121
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	TCCGTCGACTTTAGCAATCG	TTTGAACTGGTGACACGAGG	50.0	50.0	58.1	58.1	491-492 bp	1.0	80.1
trnL-UAA-trnF-GAA_p2	AGCAATCGTGAGGGTTCAAG	TTTGAACTGGTGACACGAGG	50.0	50.0	58.2	58.1	479-480 bp	1.0	79.4
trnL-UAA-trnF-GAA_p3	CCGTCGACTTTAGCAATCGT	TTTGAACTGGTGACACGAGG	50.0	50.0	58.4	58.1	490-491 bp	1.0	77.9
trnL-UAA-trnF-GAA_p4	TCCGTCGACTTTAGCAATCG	CTGCATGTCAGGAACCAGAT	50.0	50.0	58.1	57.6	510-511 bp	1.0	77.2
trnL-UAA-trnF-GAA_p5	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	476-477 bp	1.0	77.1