Hotspot

psbC-trnS-UGA

LSC · 37183-37434 · 252 bp

Back to Diphylleia

Pi 0.0278

MarkerSeek score 64.80

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 252
variable_sites: 10
pi: 0.0278
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	TCCTATTCGTGGGCCATTTG	GGTTGATAGCTCCGGTCTTG	50.0	55.0	57.9	58.1	444-445 bp	1.0	81.1
psbC-trnS-UGA_p2	TCCTATTCGTGGGCCATTTG	GATGGCCGAGTGGTTGATAG	50.0	55.0	57.9	58.1	455-456 bp	1.0	80.5
psbC-trnS-UGA_p3	TCCTATTCGTGGGCCATTTG	GGGTTCGAATCCCTCTCTCT	50.0	55.0	57.9	58.2	390-391 bp	1.0	80.1
psbC-trnS-UGA_p4	CCTATTCGTGGGCCATTTGT	GGTTGATAGCTCCGGTCTTG	50.0	55.0	58.2	58.1	443-444 bp	1.0	79.6
psbC-trnS-UGA_p5	CCTATTCGTGGGCCATTTGT	GATGGCCGAGTGGTTGATAG	50.0	55.0	58.2	58.1	454-455 bp	1.0	79.0