Hotspot

trnT-GGU-psbD

LSC · 33120-34751 · 1632 bp

Back to Diphylleia

Pi 0.011

MarkerSeek score 64.90

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1632
variable_sites: 27
pi: 0.011
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	CAAAAACGAAACGGTCCCTG	55.0	50.0	58.1	57.9	1780-1805 bp	1.0	79.8
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	CAAAAACGAAACGGTCCCTG	50.0	50.0	58.1	57.9	1783-1808 bp	1.0	79.6
trnT-GGU-psbD_p3	AACGGGCCCTTTTAACTCAG	CAAAAACGAAACGGTCCCTG	50.0	50.0	58.1	57.9	1799-1824 bp	1.0	79.5
trnT-GGU-psbD_p4	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	55.0	45.0	58.1	58.2	1781-1806 bp	1.0	78.7
trnT-GGU-psbD_p5	AACCAAACGGGCCCTTTTAA	CAAAAACGAAACGGTCCCTG	45.0	50.0	58.2	57.9	1804-1829 bp	1.0	78.6