Hotspot

petN-psbM

LSC · 29224-30532 · 1309 bp

Back to Diphylleia

Pi 0.00561
MarkerSeek score 62.50
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
1309
variable_sites
10
pi
0.00561
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 TCTTGCTTGGGCTGCTTTAA CGTCAAAACGGTCAGTCAAA 45.0 45.0 58.0 56.9 1020-1413 bp 1.0 75.8
petN-psbM_p2 AGTAAGTCTTGCTTGGGCTG CGTCAAAACGGTCAGTCAAA 50.0 45.0 57.8 56.9 1026-1419 bp 1.0 74.3
petN-psbM_p3 TCTTGCTTGGGCTGCTTTAA CGTCAAAACGGTCAGTCAAAA 45.0 42.9 58.0 57.6 1020-1413 bp 1.0 73.4
petN-psbM_p4 ATGGGGAAGAAGTGGACTCT CGTCAAAACGGTCAGTCAAA 50.0 45.0 57.6 56.9 966-1359 bp 1.0 73.1
petN-psbM_p5 AGTAAGTCTTGCTTGGGCTG CGTCAAAACGGTCAGTCAAAA 50.0 42.9 57.8 57.6 1026-1419 bp 1.0 72.0