Hotspot

trnC-GCA-petN

LSC · 28274-29127 · 854 bp

Back to Diphylleia

Pi 0.00782

MarkerSeek score 64.60

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 854
variable_sites: 10
pi: 0.00782
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.1	58.0	920-934 bp	1.0	81.4
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	CAGCCCAAGCAAGACTTACT	50.0	50.0	58.1	57.8	914-928 bp	1.0	79.9
trnC-GCA-petN_p3	GGGTGTCGCCTGATCAATAA	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	57.7	58.0	902-916 bp	1.0	79.5
trnC-GCA-petN_p4	GGGGGACTGCAAATCCTTTT	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.4	58.0	938-952 bp	1.0	79.1
trnC-GCA-petN_p5	TTTCCCCAGTTCAAATCCGG	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.1	57.6	974-988 bp	1.0	78.5