Hotspot

rpoB-trnC-GCA

LSC · 27059-28193 · 1135 bp

Back to Diphylleia

Pi 0.014

MarkerSeek score 60.20

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1135
variable_sites: 23
pi: 0.014
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	AAACCCGGGTACTGTAGACA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.0	58.1	1233-1258 bp	1.0	81.6
rpoB-trnC-GCA_p2	TGACTAAACCCGGGTACTGT	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.0	58.1	1238-1263 bp	1.0	81.6
rpoB-trnC-GCA_p3	AACCCGGGTACTGTAGACAT	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.8	58.1	1232-1257 bp	1.0	79.8
rpoB-trnC-GCA_p4	ACCCGGGTACTGTAGACATT	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.8	58.1	1231-1256 bp	1.0	79.8
rpoB-trnC-GCA_p5	AAACCCGGGTACTGTAGACA	TTATTGATCAGGCGACACCC	50.0	50.0	58.0	57.7	1251-1276 bp	1.0	79.6