Hotspot

trnS-GCU-trnG-GCC

LSC · 8355-9026 · 672 bp

Back to Diphylleia

Pi 0.0149

MarkerSeek score 56.30

Primer available yes

Label trnS-GCU-trnG-GCC

Feature Metadata

label_name: trnS-GCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 672
variable_sites: 15
pi: 0.0149
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	AAGGAACGGAAAGAGAGGGA	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	797-800 bp	1.0	53.3
trnS-GCU-trnG-GCC_p2	ATTAGCAATCCGCCGCTTTA	ACGAATCACACTTTTACCACT	45.0	38.1	58.0	55.5	743-746 bp	1.0	53.3
trnS-GCU-trnG-GCC_p3	ACGGAAAGAGAGGGATTCGA	ACGAATCACACTTTTACCACT	50.0	38.1	58.2	55.5	792-795 bp	1.0	52.3
trnS-GCU-trnG-GCC_p4	AGGAACGGAAAGAGAGGGAT	ACGAATCACACTTTTACCACT	50.0	38.1	57.7	55.5	796-799 bp	1.0	51.5
trnS-GCU-trnG-GCC_p5	AAGGAACGGAAAGAGAGGGA	AACGAATCACACTTTTACCACT	50.0	36.4	58.0	56.2	798-801 bp	1.0	51.4