Hotspot

rps18

LSC · 72215-72829 · 615 bp

Back to Dipelta

Pi 0.0217

MarkerSeek score 64.00

Primer available yes

Label rps18

Feature Metadata

label_name: rps18
feature_type: gene
region: LSC
length_bp: 615
variable_sites: 13
pi: 0.0217
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18_p1	CGACCGAAATAGGATTTTTGGG	AATCTGCGTTTGAGTTCGGA	45.5	45.0	57.7	57.8	714-720 bp	1.0	63.5
rps18_p2	CGACCGAAATAGGATTTTTGGG	TCTGCGTTTGAGTTCGGATT	45.5	45.0	57.7	57.8	712-718 bp	1.0	63.5
rps18_p3	CGACCGAAATAGGATTTTTGGG	CAATCTGCGTTTGAGTTCGG	45.5	50.0	57.7	57.8	715-721 bp	1.0	63.0
rps18_p4	CGACCGAAATAGGATTTTTGGG	CTGCGTTTGAGTTCGGATTG	45.5	50.0	57.7	57.8	711-717 bp	1.0	63.0
rps18_p5	CGACCGAAATAGGATTTTTGGG	GAGCAAAACCTCAATCTGCG	45.5	50.0	57.7	57.7	726-732 bp	1.0	62.7